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Ing, burning, thick white vaginal discharge in the case of yeast ; , and sometimes even some burning on urination, if end of urethra becomes irritated b ; birth control pills and wearing nylon instead of cotton underwear thought to make vaginitis more likely 2 ; 3 common types: a ; se vere itch ing, thick white discharge that looks like cot tage cheese: likely Candida, a type of yeast: over-the-counter antifungal creams miconazole nitrate Micatin or Monistat ; , and prescription cream clo tri ma zole, all effective against yeast if applied into vagina nightly for 5 to 7 nights b ; runny, slightly yellow, and smelly discharge: likely Gardnerella also known as "bacterial vaginosis" oral metronidazole e.g., Flgyl ; used as treatment c ; brown, smelly discharge: likely Trichomonas; also treated with metronidazole 3 ; yeast and Gardnerella are not transmitted by sex; Trichomonas can be transmitted by sex, though men rarely have symptoms; these three infections annoying but not dangerous b. organisms foreign to vagina may also cause infection; Gonococcus Chlamydia common external causes 1 ; each treated by different antibiotic: IM ceftriaxone Rocephin ; or PO ofloxacin for Gonococcus gonorrhea ; , doxycycline or ofloxacin for Chlamydia 2 ; diagnosis requires microscope and cultures, so no way to diagnose in field c. Pelvic Inflammatory Disease PID ; 1 ; re sults when vagi nal in fec tion spreads from the vagina into uterus.
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FELDENE see PIROXICAM FELODIPINE Brand Name s ; : Plendil Tablets, extended release: 5mg 10mg FENTANYL Brand Name s ; : Duragesic Patch 72hour ; : 25mcg hr 50mcg hr 75mcg hr 100mcg hr FERGENSOL see FERROUS SULFATE FERROUS SULFATE Brand Name s ; : Fergensol, FeSO4 Drops: 15mg elemental iron 0.6ml Tablets: 324mg FINASTERIDE Brand Name s ; : Proscar Tablets: 5mg FIORICET see BUTALBITAL ACETAMINOPHEN FIORINAL see BUTALBITAL ASPIRIN FLAGYL see METRONIDAZOLE FLAVOXATE Brand Name s ; : Urispas Tablets: 100mg FLEET ENEMA see SODIUM PHOSPHATE, DI and MONO SODIUM PHOSPHATE FLEET MINERAL OIL ENEMA see MINERAL OIL FLEET PREP KIT 1 see BISACODYL SODIUM BIPHOSPHATE SODIUM PHOSPHATE FLEXERIL see CYCLOBENZAPRINE FLONASE see FLUTICASONE FLOVENT HFA see FLUTICASONE FLUCONAZOLE Brand Name s ; : Diflucan Tablets: 100mg 150mg * Max 1 tablet with up to 3 refills for vaginal.
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The in vitro differences between hair follicle bulge stem cells and hair bulb keratinocytes Q Tao, C Photopoulos and S Lyle Pathology, Beth Israel Deaconess Medical Center, Boston, MA The goals of this study are to define the in vitro characteristics of keratinocyte stem cells, isolated from the bulge region of human hair follicles, and determine how they differ from the transit-amplifying TA ; cells from the hair bulb. We used telogen follicles, plucked from dispase-treated human scalp, to generate primary keratinocyte cultures from this stem cell-enriched area. We also dissected the bulb region of anagen follicles to obtain matrix TA keratinocytes. We then compared the in vitro properties of cell adhesion, cell migration, clonogenicity and in vitro life-span for these two populations. We observed epithelial outgrowths from the bulbs within two days of explant, consistent with the active proliferation of TA cells, while telogen outgrowths appeared between 7-10 days after explant, reflecting their normal in vivo quiescent nature. After growing for two weeks, the cells were re-plated and analyzed. Both populations formed colonies, however the stem cells from telogen follicles formed more colonies than bulb keratinocytes 147 + - 14 vs and more colonies 3 mm 41% vs 23% ; . When the cells were sub-cultured, telogen cells formed colonies until passage 6 when they only formed terminally differentiated cells, while bulb cells terminally differentiated at passage 3. On cell adhesion assays, the ratio of cells rapidly adhering to collagen IV within 10 min. was greater in stem cells vs. TA cells, 84.6% vs 55% respectively. Most strikingly, bulb keratinocytes showed a 7-fold greater mobility on migration assays than the telogen bulge stem cells .704 vs .102 microns min. ; Our results show distinct in vitro differences between stem cells from the telogen bulge and TA cells from the anagen bulb. These assays thus help to define the hair follicle stem cell phenotype and correlate with the in vivo properties of stem cells such as permanent residence within the stem cell niche, tightly adherent to the basement membrane. The assays can also now be used to determine the molecular requirements which maintain the differences between stem and TA cells and fluconazole.
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