Abstract -Free radicals are highly reactive molecules generated predominantly during cellular respiration and normal metabolism. Imbalance between cellular production of free radicals and the ability of cells to defend against them is referred to as oxidative stress OS ; . OS has been implicated as a potential conAddress correspondence to: Dr. Daniel Offen, Felsenstein Medical Research Center, Rabin Medical Center-Beilinson Campus, Petah Tikva 49100 Israel. E-mail: doffen post.tau.ac.il.
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J Acoust Soc Am. 2007 Mar; 121 3 ; : 1632-1641. Parra LC, Pearlmutter BA Biomedical Engineering Department, City College of New York, New York, NY 10031, USA. parra ccny.cuny Phenomena resembling tinnitus and Zwicker phantom tone are seen to result from an auditory gain adaptation mechanism that attempts to make full use of a fixed-capacity channel. In the case of tinnitus, the gain adaptation enhances internal noise of a frequency band otherwise silent due to damage. This generates a percept of a phantom sound as a consequence of hearing loss. In the case of Zwicker tone, a frequency band is temporarily silent during the presentation of a notched broadband sound, resulting in a percept of a tone at the notched frequency. The model suggests a link between tinnitus and the Zwicker tone percept, in that it predicts different results for normal and tinnitus subjects due to a loss of instantaneous nonlinear compression. Listening experiments on 44 subjects show that tinnitus subjects 11 of 44 ; are significantly more likely to hear the Zwicker tone. This psychoacoustic experiment establishes the first empirical link between the Zwicker tone percept and tinnitus. Together with the modeling results, this supports the hypothesis that the phantom percept is a consequence of a central adaptation mechanism confronted with a degraded sensory apparatus.
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Isolation of single human atrial myocytes Human atrial cells were isolated from human atrium pieces obtained from patients undergoing coronary bypass surgery in Kaunas Medical University Clinics. Atrial cells were enzymatically dispersed by a combination of collagenase, protease, and elastase as described by Jureviius et al. [8]. The procedure for obtaining the tissue was approved by the institutional Ethics Committee. Upon excision, the samples were immediately placed in an oxygenated cardioplegic St. Thomas solution containing in mmol l ; : 110 NaCl, 5.4 KCl, 16 MgCl2, 1.2 CaCl2, 5.5 D-glucose, 10 HEPES, pH adjusted to 7.4 with NaOH, and were transported to laboratory. Tissue specimens were cut into small pieces ~1mm3 ; and twice washed out in modified calcium-free, oxygenated 95 percent O2, 5 percent CO2 ; Tyrode solution of the following composition in mmol l ; : 10 HEPES, 35 NaCl, 10 Dglucose, 134 sucrose, 16 Na2HPO4, 25 NaHCO3, 4.7 KCl, 1.2 KH2PO4, pH 7.2 NaOH ; at room temperature 18-22oC ; . Thirty mmoles of 2, 3-butanedionmonoxime were added to the solution to protect the tissue from hypoxic injury. The tissue was then placed in 25-ml flask with 10 ml of nominally calcium-free Tyrode solution containing BSA 5mg ml; Boehringer, Mannheim ; , collagenase 200 U ml, type 2; Worthington ; , protease 6 U ml, type XXIV; SIGMA ; and elastase 13 U ml; Boehringer Mannheim ; and agitated for 30 min. Afterwards, the solution was replaced by 10 ml fresh enzymatic solution containing only collagenase 400 U ml; Worthington ; . After 15-20 min of agitation, the digestive solution was replaced by a fresh one with collagenase 400 U ml ; and the agitation was and premphase.
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