One-week-old culture of A. diazotrophicus grown on modified LGIP medium was suspended in sterile distilled water and its 1% suspension was added to the same melted medium. The medium was shaken thorougly and poured in thin layers into sterile petri plates and allowed to solidify. Paper discs of 10 mm diameter were soaked in different concentrations of antibiotics 0, 40, 80, 120, and 200 g ; and placed on the same solidified agar medium. The petri plates were incubated at 29C for 1 week and observed for inhibition zones around the paper discs for 7 days. A paper disc treated with sterile distilled water served as control. Simultaneously, the effect of different concentrations of the same antibiotics on the population and growth curve of A. diazotrophicus was assessed by agar dilution method. Plates of modified LGIP medium with different concentrations of various antibiotics 0, 40, 80, 120, and 200 g ; were prepared. About 1 104 Acetobacter cells 1 ml plate ; were spotted and incubated at 29C for 1 week. The total viable count was estimated in terms of TVC ml at an interval of 24 h. The study of cultural characteristics and Gram staining showed that the isolate was slow growing, appeared as orange-coloured colony on modified LGIP medium and Gram-negative rods Figure 1 ; . The P-solubilizing capacity of the isolated strain of A. diazotrophicus was measured after 7 days of incubation. It was found to be 20.5 g 15 mg insoluble P per 0.15 g of sucrose consumed after 7 days of incubation. Further, there was a pH drop from initial 6.8 to 3.0, since it ferments the sugar and reduces the pH of the medium3, 6. The strain also showed the good solubilization zone on Pikovskaya medium supplemented with insoluble tricalcium phosphate, indicating its potential role as a P-solubilizer. Antibiotics resistance study and growth curve of this particular strain with different concentrations of antibiotics revealed that the bacterium was fully resistant to erythromycin, ampicillin and roxithromycin, irrespective of any concentration Table 1, Figure 2 ; . The result further showed that the bacterium was found to be resistant to penicillin and tetracycline up to 80 and 120 g, respectively. The inhibition zones of 0.1 and 0.2 cm were observed at 120 g and 160 g concentration of penicillin and tetracyclin, respectively. However, the bacterium was found to be sensitive to the other antibiotics like ciprofoxacin, chloramphenicol, dexycycline, streptomycin and rifampicin at all the concentra. Side effects and toxicity there have been very few reports of side effects when being treated with roxithromycin.

GENERAL PROVISIONS Continued ; PAYMENT OF PREMIUM: All premiums are payable in advance for each policy term in accordance with the Company's premium rates. The full premium must be paid even if the premium is received after the policy Effective Date. There is no pro-rata or reduced premium payment for late enrollees. There will be no refunds to students who cancel coverage under the policy; unless the Insured enters the armed forces. Premium adjustments involving return of unearned premiums to the Policyholder will be limited to a period of 12 months immediately preceding the date of receipt by the Company of evidence that adjustments should be made. Premiums are payable to the Company, P.O. Box 809067, Dallas, Texas 75380-9067. NOTICE OF CLAIM: Written notice of claim must be given to the Company within thirty 30 ; days after the occurrence or commencement of any loss covered by this policy, or as soon thereafter as is reasonably possible. Notice given by or on behalf of the Named Insured to the Company, P.O. Box 809067, Dallas, Texas 75380-9067 with information sufficient to identify the Named Insured shall be deemed notice to the Company. CLAIM FORMS: Claim forms are not required. PROOF OF LOSS: Written proof of loss must be furnished to the Company at its said office within 90 days after the date of such loss. Failure to furnish such proof within the time required will not invalidate nor reduce any claim if it was not reasonably possible to furnish proof. In no event except in the absence of legal capacity shall written proofs of loss be furnished later than one year from the time proof is otherwise required. TIME OF PAYMENT OF CLAIM: Indemnities payable under this policy for any loss will be paid within twenty-five 25 ; days after receipt of due written proof of such loss in the form of a Clean Claim where claims are submitted electronically, and will be paid within thirty-five 35 ; days after receipt of due written proof of such loss in the form of Clean Claim where claims are submitted in paper format. Benefits due under the policy and claims are overdue if not paid within twenty-five 25 ; days or thirty-five 35 ; days, whichever is applicable after the Company receives a Clean Claim containing necessary medical information and other information essential for the Company to administer Pre-Existing Condition, Coordination of Benefits and Subrogation provisions. A "clean claim" means a claim received by the Company for adjudication and which requires no further information, adjustment or alteration by the provider of the services or the Insured in order to be processed and paid by the Company. A claim is clean if it has no defect or impropriety, including any lack of substantiating documentation, or particular circumstance requiring special treatment that prevents timely payment from being made on the claim under this provision. A Clean Claim includes resubmitted claims with previously identified deficiencies corrected. A Clean Claim does not include any of the following: a ; a duplicate claim, which means an original claim and its duplicate when the duplicate is filed within thirty 30 ; days of the original claim; b ; claims which are submitted fraudulently or that are based upon material misrepresentations; c ; claims that require information essential for the Company to administer PreExisting Condition, Coordination of Benefits or Subrogation provisions; or d ; claims submitted by a provider more than thirty 30 ; days after the date of service; if the provider does not submit the claim on behalf of the Insured, then a claim is not clean when submitted more than thirty 30 ; days after the date of billing by the provider to the Insured. Not later than twenty-five 25 ; days after the date the Company actually receives an electronic claim, the Company shall pay the appropriate benefit in full, or any portion of the claim that is clean, and notify the provider where the claim is owed to the provider ; or the Insured where the claim is owed to the Insured ; of the reasons why the claim or portion thereof is not clean and will not be paid and what substantiating documentation and information is required to adjudicate the claim as clean. Not later than thirty-five 35 ; days after the date the Company actually receives a paper claim, the Company shall pay the appropriate benefit in full, or any portion of the claim that is clean, and notify the provider where the claim is owed to the provider ; or the Insured where the claim is owed to the Insured ; of the reasons why the claim or portion thereof is not clean and will not be paid and what substantiating documentation and information is required to adjudicate the claim as clean. Any claim or portion thereof resubmitted with the supporting documentation and information requested by the Company shall be paid within twenty 20 ; days after receipt. View more  » roxithromycin wikipedia : roxithromycin is a semi-synthetic macrolide antibiotic.
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